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KMID : 1098420220300030210
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Korean Journal of Medicinal Crop Science
2022 Volume.30 No. 3 p.210 ~ p.216
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Development of InDel Markers to Distinguish between Prunus mume, Prunus armeniaca var. ansu, and their Interspecific Hybrids
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Kim Ju-Hyeok
Lee Dong-Wook
Kim Moon-Kyo
Lee Mi-Sun
Jo Nam-Su
Lee Yi
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Abstract
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Background: Prunus mume Sieb. et. Zucc. is an important medicinal resource, and its fruit is used as a health food worldwide. However, this plant is often confused with Prunus armeniaca var.
ansu., a related but distinct deciduous species. To distinguish between these plants, we developed co-dominant insertion-deletion (InDel) markers.
Methods and Results: DNA was extracted from P. mume and P. armeniaca leaves and subjected to next-generation sequencing (NGS) analysis. After assembly, 30 polymorphic loci were identi?fied. Primers were designed for the polymorphic loci and used to genotype P. mume and P. armeni?aca. Using two InDel markers developed from the polymorphic loci, we distinguished between P.
mume and P. armeniaca, and also identified the genetic hybrids between P. mume and P. armeni?aca.
Conclusions: The two markers developed in this study can be used to authenticate P. mume, P.
armeniaca, and their hybrids.
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KEYWORD
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Prunus mume, Prunus armeniaca var. ansu, Genotyping, Insertion-Deletion, Next?Generation Sequencing
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